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anti irf7 rabbit polyclonal antibody pab  (Bioss)


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    Bioss anti irf7 rabbit polyclonal antibody pab
    Anti Irf7 Rabbit Polyclonal Antibody Pab, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+irf7+rabbit+polyclonal+antibody+pab/IRF7+Polyclonal+Antibody/pmc12968265-59-7-13
    Average 94 stars, based on 5 article reviews
    anti irf7 rabbit polyclonal antibody pab - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Sequencing:

    Article Title: Chicken caspase-3 promotes IBDV replication via the cleavage of IRF7
    Article Snippet: Anti-IBDV VP2 mouse monoclonal antibody (mAb) was prepared in our laboratory.Anti-IBDV VP2 mouse monoclonal antibody (mAb) was prepared in our laboratory.. Other antibodies used in our study were anti-IRF7 rabbit polyclonal antibody (pAb) (bs-2994R, BIOSS, China), anti-Caspase-3 (Pro and Active) mouse mAb (31A1067, Novus, United States), anti-β-actin mouse mAb (CW0096M, CWBIO, China), anti-His mouse mAb ( M20001 , Abmart, China), anti-HA mouse mAb ( M20003 , Abmart, China), goat anti-rabbit IgG H&L antibody (CW0103S, CWBIO, China), goat anti-mouse IgG H&L antibody (CW0102S, CWBIO, China), AbBox Fluor 594-labeled goat anti-rabbit IgG (BD9279, Biodragon, China), FITC-labeled goat anti-mouse IgG (BF05001, Biodragon, China).. Other reagents were the caspase inhibitor Z-VAD (HY-16658B, MedChemExpress, United States), Caspase-3 inhibitor Z-DEVD (HY-12466, MedChemExpress, United States), SYBR Green qPCR Mix (11201ES, YEASEN, China), Cell Counting Kit-8 (C6005, NCM Biotech, China), Annexin V-FITC Apoptosis Detection Kit (556547, BD Biosciences, United States), Lipo8000 transfection reagent (C0533, Beyotime, China), RIPA buffer (P0013B, Beyotime, China), PBS (C0221A, Beyotime, China), Protease Inhibitor Cocktail (CW2200S, CWBIO, China), 5 × SDS loading buffer (CW0027S, CWBIO, China), TRIzon Reagent (CW0580, CWBIO, China), HiFiScript gDNA Removal RT MasterMix (CW2020M, CWBIO, China), protein A/G agarose (80104G, Invivogen, FR).Other reagents were the caspase inhibitor Z-VAD (HY-16658B, MedChemExpress, United States), Caspase-3 inhibitor Z-DEVD (HY-12466, MedChemExpress, United States), SYBR Green qPCR Mix (11201ES, YEASEN, China), Cell..

    Article Title: Infectious bursal disease virus affecting interferon regulatory factor 7 signaling through VP3 protein to facilitate viral replication
    Article Snippet: VP2 mouse monoclonal antibody (mAb) was prepared in our laboratory. .. Other antibodies used in our study were anti-IRF7 rabbit polyclonal antibody (pAb) (bs-2994R, BIOSS, China), anti-FLAG mouse mAb (CW0287, CWBIO, China), anti-β-actin mouse mAb (CW0096M, CWBIO, China), anti-His mouse mAb (M20001M, Abmart, China), AbBox Fluor 594-labeled goat anti-rabbit IgG (BD9279, Biodragon, China), FITC-labeled goat anti-mouse IgG (BF05001, Biodragon, China), goat anti-rabbit IgG H&L antibody (CW0103S, CWBIO, China), goat anti-mouse IgG H&L antibody (CW0102S, CWBIO, China). .. Other reagents were the proteasome inhibitor MG132 (A2585, APEXBIO, China), u

    Infection:

    Article Title: Chicken caspase-3 promotes IBDV replication via the cleavage of IRF7
    Article Snippet: Anti-IBDV VP2 mouse monoclonal antibody (mAb) was prepared in our laboratory.Anti-IBDV VP2 mouse monoclonal antibody (mAb) was prepared in our laboratory.. Other antibodies used in our study were anti-IRF7 rabbit polyclonal antibody (pAb) (bs-2994R, BIOSS, China), anti-Caspase-3 (Pro and Active) mouse mAb (31A1067, Novus, United States), anti-β-actin mouse mAb (CW0096M, CWBIO, China), anti-His mouse mAb ( M20001 , Abmart, China), anti-HA mouse mAb ( M20003 , Abmart, China), goat anti-rabbit IgG H&L antibody (CW0103S, CWBIO, China), goat anti-mouse IgG H&L antibody (CW0102S, CWBIO, China), AbBox Fluor 594-labeled goat anti-rabbit IgG (BD9279, Biodragon, China), FITC-labeled goat anti-mouse IgG (BF05001, Biodragon, China).. Other reagents were the caspase inhibitor Z-VAD (HY-16658B, MedChemExpress, United States), Caspase-3 inhibitor Z-DEVD (HY-12466, MedChemExpress, United States), SYBR Green qPCR Mix (11201ES, YEASEN, China), Cell Counting Kit-8 (C6005, NCM Biotech, China), Annexin V-FITC Apoptosis Detection Kit (556547, BD Biosciences, United States), Lipo8000 transfection reagent (C0533, Beyotime, China), RIPA buffer (P0013B, Beyotime, China), PBS (C0221A, Beyotime, China), Protease Inhibitor Cocktail (CW2200S, CWBIO, China), 5 × SDS loading buffer (CW0027S, CWBIO, China), TRIzon Reagent (CW0580, CWBIO, China), HiFiScript gDNA Removal RT MasterMix (CW2020M, CWBIO, China), protein A/G agarose (80104G, Invivogen, FR).Other reagents were the caspase inhibitor Z-VAD (HY-16658B, MedChemExpress, United States), Caspase-3 inhibitor Z-DEVD (HY-12466, MedChemExpress, United States), SYBR Green qPCR Mix (11201ES, YEASEN, China), Cell..

    Article Title: Infectious bursal disease virus affecting interferon regulatory factor 7 signaling through VP3 protein to facilitate viral replication
    Article Snippet: VP2 mouse monoclonal antibody (mAb) was prepared in our laboratory. .. Other antibodies used in our study were anti-IRF7 rabbit polyclonal antibody (pAb) (bs-2994R, BIOSS, China), anti-FLAG mouse mAb (CW0287, CWBIO, China), anti-β-actin mouse mAb (CW0096M, CWBIO, China), anti-His mouse mAb (M20001M, Abmart, China), AbBox Fluor 594-labeled goat anti-rabbit IgG (BD9279, Biodragon, China), FITC-labeled goat anti-mouse IgG (BF05001, Biodragon, China), goat anti-rabbit IgG H&L antibody (CW0103S, CWBIO, China), goat anti-mouse IgG H&L antibody (CW0102S, CWBIO, China). .. Other reagents were the proteasome inhibitor MG132 (A2585, APEXBIO, China), u

    Expressing:

    Article Title: Chicken caspase-3 promotes IBDV replication via the cleavage of IRF7
    Article Snippet: Anti-IBDV VP2 mouse monoclonal antibody (mAb) was prepared in our laboratory.Anti-IBDV VP2 mouse monoclonal antibody (mAb) was prepared in our laboratory.. Other antibodies used in our study were anti-IRF7 rabbit polyclonal antibody (pAb) (bs-2994R, BIOSS, China), anti-Caspase-3 (Pro and Active) mouse mAb (31A1067, Novus, United States), anti-β-actin mouse mAb (CW0096M, CWBIO, China), anti-His mouse mAb ( M20001 , Abmart, China), anti-HA mouse mAb ( M20003 , Abmart, China), goat anti-rabbit IgG H&L antibody (CW0103S, CWBIO, China), goat anti-mouse IgG H&L antibody (CW0102S, CWBIO, China), AbBox Fluor 594-labeled goat anti-rabbit IgG (BD9279, Biodragon, China), FITC-labeled goat anti-mouse IgG (BF05001, Biodragon, China).. Other reagents were the caspase inhibitor Z-VAD (HY-16658B, MedChemExpress, United States), Caspase-3 inhibitor Z-DEVD (HY-12466, MedChemExpress, United States), SYBR Green qPCR Mix (11201ES, YEASEN, China), Cell Counting Kit-8 (C6005, NCM Biotech, China), Annexin V-FITC Apoptosis Detection Kit (556547, BD Biosciences, United States), Lipo8000 transfection reagent (C0533, Beyotime, China), RIPA buffer (P0013B, Beyotime, China), PBS (C0221A, Beyotime, China), Protease Inhibitor Cocktail (CW2200S, CWBIO, China), 5 × SDS loading buffer (CW0027S, CWBIO, China), TRIzon Reagent (CW0580, CWBIO, China), HiFiScript gDNA Removal RT MasterMix (CW2020M, CWBIO, China), protein A/G agarose (80104G, Invivogen, FR).Other reagents were the caspase inhibitor Z-VAD (HY-16658B, MedChemExpress, United States), Caspase-3 inhibitor Z-DEVD (HY-12466, MedChemExpress, United States), SYBR Green qPCR Mix (11201ES, YEASEN, China), Cell..

    Article Title: Infectious bursal disease virus affecting interferon regulatory factor 7 signaling through VP3 protein to facilitate viral replication
    Article Snippet: VP2 mouse monoclonal antibody (mAb) was prepared in our laboratory. .. Other antibodies used in our study were anti-IRF7 rabbit polyclonal antibody (pAb) (bs-2994R, BIOSS, China), anti-FLAG mouse mAb (CW0287, CWBIO, China), anti-β-actin mouse mAb (CW0096M, CWBIO, China), anti-His mouse mAb (M20001M, Abmart, China), AbBox Fluor 594-labeled goat anti-rabbit IgG (BD9279, Biodragon, China), FITC-labeled goat anti-mouse IgG (BF05001, Biodragon, China), goat anti-rabbit IgG H&L antibody (CW0103S, CWBIO, China), goat anti-mouse IgG H&L antibody (CW0102S, CWBIO, China). .. Other reagents were the proteasome inhibitor MG132 (A2585, APEXBIO, China), u

    Quantitative RT-PCR:

    Article Title: Chicken caspase-3 promotes IBDV replication via the cleavage of IRF7
    Article Snippet: Anti-IBDV VP2 mouse monoclonal antibody (mAb) was prepared in our laboratory.Anti-IBDV VP2 mouse monoclonal antibody (mAb) was prepared in our laboratory.. Other antibodies used in our study were anti-IRF7 rabbit polyclonal antibody (pAb) (bs-2994R, BIOSS, China), anti-Caspase-3 (Pro and Active) mouse mAb (31A1067, Novus, United States), anti-β-actin mouse mAb (CW0096M, CWBIO, China), anti-His mouse mAb ( M20001 , Abmart, China), anti-HA mouse mAb ( M20003 , Abmart, China), goat anti-rabbit IgG H&L antibody (CW0103S, CWBIO, China), goat anti-mouse IgG H&L antibody (CW0102S, CWBIO, China), AbBox Fluor 594-labeled goat anti-rabbit IgG (BD9279, Biodragon, China), FITC-labeled goat anti-mouse IgG (BF05001, Biodragon, China).. Other reagents were the caspase inhibitor Z-VAD (HY-16658B, MedChemExpress, United States), Caspase-3 inhibitor Z-DEVD (HY-12466, MedChemExpress, United States), SYBR Green qPCR Mix (11201ES, YEASEN, China), Cell Counting Kit-8 (C6005, NCM Biotech, China), Annexin V-FITC Apoptosis Detection Kit (556547, BD Biosciences, United States), Lipo8000 transfection reagent (C0533, Beyotime, China), RIPA buffer (P0013B, Beyotime, China), PBS (C0221A, Beyotime, China), Protease Inhibitor Cocktail (CW2200S, CWBIO, China), 5 × SDS loading buffer (CW0027S, CWBIO, China), TRIzon Reagent (CW0580, CWBIO, China), HiFiScript gDNA Removal RT MasterMix (CW2020M, CWBIO, China), protein A/G agarose (80104G, Invivogen, FR).Other reagents were the caspase inhibitor Z-VAD (HY-16658B, MedChemExpress, United States), Caspase-3 inhibitor Z-DEVD (HY-12466, MedChemExpress, United States), SYBR Green qPCR Mix (11201ES, YEASEN, China), Cell..

    Article Title: Infectious bursal disease virus affecting interferon regulatory factor 7 signaling through VP3 protein to facilitate viral replication
    Article Snippet: VP2 mouse monoclonal antibody (mAb) was prepared in our laboratory. .. Other antibodies used in our study were anti-IRF7 rabbit polyclonal antibody (pAb) (bs-2994R, BIOSS, China), anti-FLAG mouse mAb (CW0287, CWBIO, China), anti-β-actin mouse mAb (CW0096M, CWBIO, China), anti-His mouse mAb (M20001M, Abmart, China), AbBox Fluor 594-labeled goat anti-rabbit IgG (BD9279, Biodragon, China), FITC-labeled goat anti-mouse IgG (BF05001, Biodragon, China), goat anti-rabbit IgG H&L antibody (CW0103S, CWBIO, China), goat anti-mouse IgG H&L antibody (CW0102S, CWBIO, China). .. Other reagents were the proteasome inhibitor MG132 (A2585, APEXBIO, China), u

    Western Blot:

    Article Title: Chicken caspase-3 promotes IBDV replication via the cleavage of IRF7
    Article Snippet: Anti-IBDV VP2 mouse monoclonal antibody (mAb) was prepared in our laboratory.Anti-IBDV VP2 mouse monoclonal antibody (mAb) was prepared in our laboratory.. Other antibodies used in our study were anti-IRF7 rabbit polyclonal antibody (pAb) (bs-2994R, BIOSS, China), anti-Caspase-3 (Pro and Active) mouse mAb (31A1067, Novus, United States), anti-β-actin mouse mAb (CW0096M, CWBIO, China), anti-His mouse mAb ( M20001 , Abmart, China), anti-HA mouse mAb ( M20003 , Abmart, China), goat anti-rabbit IgG H&L antibody (CW0103S, CWBIO, China), goat anti-mouse IgG H&L antibody (CW0102S, CWBIO, China), AbBox Fluor 594-labeled goat anti-rabbit IgG (BD9279, Biodragon, China), FITC-labeled goat anti-mouse IgG (BF05001, Biodragon, China).. Other reagents were the caspase inhibitor Z-VAD (HY-16658B, MedChemExpress, United States), Caspase-3 inhibitor Z-DEVD (HY-12466, MedChemExpress, United States), SYBR Green qPCR Mix (11201ES, YEASEN, China), Cell Counting Kit-8 (C6005, NCM Biotech, China), Annexin V-FITC Apoptosis Detection Kit (556547, BD Biosciences, United States), Lipo8000 transfection reagent (C0533, Beyotime, China), RIPA buffer (P0013B, Beyotime, China), PBS (C0221A, Beyotime, China), Protease Inhibitor Cocktail (CW2200S, CWBIO, China), 5 × SDS loading buffer (CW0027S, CWBIO, China), TRIzon Reagent (CW0580, CWBIO, China), HiFiScript gDNA Removal RT MasterMix (CW2020M, CWBIO, China), protein A/G agarose (80104G, Invivogen, FR).Other reagents were the caspase inhibitor Z-VAD (HY-16658B, MedChemExpress, United States), Caspase-3 inhibitor Z-DEVD (HY-12466, MedChemExpress, United States), SYBR Green qPCR Mix (11201ES, YEASEN, China), Cell..

    Article Title: Infectious bursal disease virus affecting interferon regulatory factor 7 signaling through VP3 protein to facilitate viral replication
    Article Snippet: VP2 mouse monoclonal antibody (mAb) was prepared in our laboratory. .. Other antibodies used in our study were anti-IRF7 rabbit polyclonal antibody (pAb) (bs-2994R, BIOSS, China), anti-FLAG mouse mAb (CW0287, CWBIO, China), anti-β-actin mouse mAb (CW0096M, CWBIO, China), anti-His mouse mAb (M20001M, Abmart, China), AbBox Fluor 594-labeled goat anti-rabbit IgG (BD9279, Biodragon, China), FITC-labeled goat anti-mouse IgG (BF05001, Biodragon, China), goat anti-rabbit IgG H&L antibody (CW0103S, CWBIO, China), goat anti-mouse IgG H&L antibody (CW0102S, CWBIO, China). .. Other reagents were the proteasome inhibitor MG132 (A2585, APEXBIO, China), u

    Standard Deviation:

    Article Title: Chicken caspase-3 promotes IBDV replication via the cleavage of IRF7
    Article Snippet: Anti-IBDV VP2 mouse monoclonal antibody (mAb) was prepared in our laboratory.Anti-IBDV VP2 mouse monoclonal antibody (mAb) was prepared in our laboratory.. Other antibodies used in our study were anti-IRF7 rabbit polyclonal antibody (pAb) (bs-2994R, BIOSS, China), anti-Caspase-3 (Pro and Active) mouse mAb (31A1067, Novus, United States), anti-β-actin mouse mAb (CW0096M, CWBIO, China), anti-His mouse mAb ( M20001 , Abmart, China), anti-HA mouse mAb ( M20003 , Abmart, China), goat anti-rabbit IgG H&L antibody (CW0103S, CWBIO, China), goat anti-mouse IgG H&L antibody (CW0102S, CWBIO, China), AbBox Fluor 594-labeled goat anti-rabbit IgG (BD9279, Biodragon, China), FITC-labeled goat anti-mouse IgG (BF05001, Biodragon, China).. Other reagents were the caspase inhibitor Z-VAD (HY-16658B, MedChemExpress, United States), Caspase-3 inhibitor Z-DEVD (HY-12466, MedChemExpress, United States), SYBR Green qPCR Mix (11201ES, YEASEN, China), Cell Counting Kit-8 (C6005, NCM Biotech, China), Annexin V-FITC Apoptosis Detection Kit (556547, BD Biosciences, United States), Lipo8000 transfection reagent (C0533, Beyotime, China), RIPA buffer (P0013B, Beyotime, China), PBS (C0221A, Beyotime, China), Protease Inhibitor Cocktail (CW2200S, CWBIO, China), 5 × SDS loading buffer (CW0027S, CWBIO, China), TRIzon Reagent (CW0580, CWBIO, China), HiFiScript gDNA Removal RT MasterMix (CW2020M, CWBIO, China), protein A/G agarose (80104G, Invivogen, FR).Other reagents were the caspase inhibitor Z-VAD (HY-16658B, MedChemExpress, United States), Caspase-3 inhibitor Z-DEVD (HY-12466, MedChemExpress, United States), SYBR Green qPCR Mix (11201ES, YEASEN, China), Cell..

    Article Title: Infectious bursal disease virus affecting interferon regulatory factor 7 signaling through VP3 protein to facilitate viral replication
    Article Snippet: VP2 mouse monoclonal antibody (mAb) was prepared in our laboratory. .. Other antibodies used in our study were anti-IRF7 rabbit polyclonal antibody (pAb) (bs-2994R, BIOSS, China), anti-FLAG mouse mAb (CW0287, CWBIO, China), anti-β-actin mouse mAb (CW0096M, CWBIO, China), anti-His mouse mAb (M20001M, Abmart, China), AbBox Fluor 594-labeled goat anti-rabbit IgG (BD9279, Biodragon, China), FITC-labeled goat anti-mouse IgG (BF05001, Biodragon, China), goat anti-rabbit IgG H&L antibody (CW0103S, CWBIO, China), goat anti-mouse IgG H&L antibody (CW0102S, CWBIO, China). .. Other reagents were the proteasome inhibitor MG132 (A2585, APEXBIO, China), u

    Over Expression:

    Article Title: Chicken caspase-3 promotes IBDV replication via the cleavage of IRF7
    Article Snippet: Anti-IBDV VP2 mouse monoclonal antibody (mAb) was prepared in our laboratory.Anti-IBDV VP2 mouse monoclonal antibody (mAb) was prepared in our laboratory.. Other antibodies used in our study were anti-IRF7 rabbit polyclonal antibody (pAb) (bs-2994R, BIOSS, China), anti-Caspase-3 (Pro and Active) mouse mAb (31A1067, Novus, United States), anti-β-actin mouse mAb (CW0096M, CWBIO, China), anti-His mouse mAb ( M20001 , Abmart, China), anti-HA mouse mAb ( M20003 , Abmart, China), goat anti-rabbit IgG H&L antibody (CW0103S, CWBIO, China), goat anti-mouse IgG H&L antibody (CW0102S, CWBIO, China), AbBox Fluor 594-labeled goat anti-rabbit IgG (BD9279, Biodragon, China), FITC-labeled goat anti-mouse IgG (BF05001, Biodragon, China).. Other reagents were the caspase inhibitor Z-VAD (HY-16658B, MedChemExpress, United States), Caspase-3 inhibitor Z-DEVD (HY-12466, MedChemExpress, United States), SYBR Green qPCR Mix (11201ES, YEASEN, China), Cell Counting Kit-8 (C6005, NCM Biotech, China), Annexin V-FITC Apoptosis Detection Kit (556547, BD Biosciences, United States), Lipo8000 transfection reagent (C0533, Beyotime, China), RIPA buffer (P0013B, Beyotime, China), PBS (C0221A, Beyotime, China), Protease Inhibitor Cocktail (CW2200S, CWBIO, China), 5 × SDS loading buffer (CW0027S, CWBIO, China), TRIzon Reagent (CW0580, CWBIO, China), HiFiScript gDNA Removal RT MasterMix (CW2020M, CWBIO, China), protein A/G agarose (80104G, Invivogen, FR).Other reagents were the caspase inhibitor Z-VAD (HY-16658B, MedChemExpress, United States), Caspase-3 inhibitor Z-DEVD (HY-12466, MedChemExpress, United States), SYBR Green qPCR Mix (11201ES, YEASEN, China), Cell..

    Article Title: Infectious bursal disease virus affecting interferon regulatory factor 7 signaling through VP3 protein to facilitate viral replication
    Article Snippet: VP2 mouse monoclonal antibody (mAb) was prepared in our laboratory. .. Other antibodies used in our study were anti-IRF7 rabbit polyclonal antibody (pAb) (bs-2994R, BIOSS, China), anti-FLAG mouse mAb (CW0287, CWBIO, China), anti-β-actin mouse mAb (CW0096M, CWBIO, China), anti-His mouse mAb (M20001M, Abmart, China), AbBox Fluor 594-labeled goat anti-rabbit IgG (BD9279, Biodragon, China), FITC-labeled goat anti-mouse IgG (BF05001, Biodragon, China), goat anti-rabbit IgG H&L antibody (CW0103S, CWBIO, China), goat anti-mouse IgG H&L antibody (CW0102S, CWBIO, China). .. Other reagents were the proteasome inhibitor MG132 (A2585, APEXBIO, China), u

    Plasmid Preparation:

    Article Title: Chicken caspase-3 promotes IBDV replication via the cleavage of IRF7
    Article Snippet: Anti-IBDV VP2 mouse monoclonal antibody (mAb) was prepared in our laboratory.Anti-IBDV VP2 mouse monoclonal antibody (mAb) was prepared in our laboratory.. Other antibodies used in our study were anti-IRF7 rabbit polyclonal antibody (pAb) (bs-2994R, BIOSS, China), anti-Caspase-3 (Pro and Active) mouse mAb (31A1067, Novus, United States), anti-β-actin mouse mAb (CW0096M, CWBIO, China), anti-His mouse mAb ( M20001 , Abmart, China), anti-HA mouse mAb ( M20003 , Abmart, China), goat anti-rabbit IgG H&L antibody (CW0103S, CWBIO, China), goat anti-mouse IgG H&L antibody (CW0102S, CWBIO, China), AbBox Fluor 594-labeled goat anti-rabbit IgG (BD9279, Biodragon, China), FITC-labeled goat anti-mouse IgG (BF05001, Biodragon, China).. Other reagents were the caspase inhibitor Z-VAD (HY-16658B, MedChemExpress, United States), Caspase-3 inhibitor Z-DEVD (HY-12466, MedChemExpress, United States), SYBR Green qPCR Mix (11201ES, YEASEN, China), Cell Counting Kit-8 (C6005, NCM Biotech, China), Annexin V-FITC Apoptosis Detection Kit (556547, BD Biosciences, United States), Lipo8000 transfection reagent (C0533, Beyotime, China), RIPA buffer (P0013B, Beyotime, China), PBS (C0221A, Beyotime, China), Protease Inhibitor Cocktail (CW2200S, CWBIO, China), 5 × SDS loading buffer (CW0027S, CWBIO, China), TRIzon Reagent (CW0580, CWBIO, China), HiFiScript gDNA Removal RT MasterMix (CW2020M, CWBIO, China), protein A/G agarose (80104G, Invivogen, FR).Other reagents were the caspase inhibitor Z-VAD (HY-16658B, MedChemExpress, United States), Caspase-3 inhibitor Z-DEVD (HY-12466, MedChemExpress, United States), SYBR Green qPCR Mix (11201ES, YEASEN, China), Cell..

    Article Title: Infectious bursal disease virus affecting interferon regulatory factor 7 signaling through VP3 protein to facilitate viral replication
    Article Snippet: VP2 mouse monoclonal antibody (mAb) was prepared in our laboratory. .. Other antibodies used in our study were anti-IRF7 rabbit polyclonal antibody (pAb) (bs-2994R, BIOSS, China), anti-FLAG mouse mAb (CW0287, CWBIO, China), anti-β-actin mouse mAb (CW0096M, CWBIO, China), anti-His mouse mAb (M20001M, Abmart, China), AbBox Fluor 594-labeled goat anti-rabbit IgG (BD9279, Biodragon, China), FITC-labeled goat anti-mouse IgG (BF05001, Biodragon, China), goat anti-rabbit IgG H&L antibody (CW0103S, CWBIO, China), goat anti-mouse IgG H&L antibody (CW0102S, CWBIO, China). .. Other reagents were the proteasome inhibitor MG132 (A2585, APEXBIO, China), u

    Transfection:

    Article Title: Chicken caspase-3 promotes IBDV replication via the cleavage of IRF7
    Article Snippet: Anti-IBDV VP2 mouse monoclonal antibody (mAb) was prepared in our laboratory.Anti-IBDV VP2 mouse monoclonal antibody (mAb) was prepared in our laboratory.. Other antibodies used in our study were anti-IRF7 rabbit polyclonal antibody (pAb) (bs-2994R, BIOSS, China), anti-Caspase-3 (Pro and Active) mouse mAb (31A1067, Novus, United States), anti-β-actin mouse mAb (CW0096M, CWBIO, China), anti-His mouse mAb ( M20001 , Abmart, China), anti-HA mouse mAb ( M20003 , Abmart, China), goat anti-rabbit IgG H&L antibody (CW0103S, CWBIO, China), goat anti-mouse IgG H&L antibody (CW0102S, CWBIO, China), AbBox Fluor 594-labeled goat anti-rabbit IgG (BD9279, Biodragon, China), FITC-labeled goat anti-mouse IgG (BF05001, Biodragon, China).. Other reagents were the caspase inhibitor Z-VAD (HY-16658B, MedChemExpress, United States), Caspase-3 inhibitor Z-DEVD (HY-12466, MedChemExpress, United States), SYBR Green qPCR Mix (11201ES, YEASEN, China), Cell Counting Kit-8 (C6005, NCM Biotech, China), Annexin V-FITC Apoptosis Detection Kit (556547, BD Biosciences, United States), Lipo8000 transfection reagent (C0533, Beyotime, China), RIPA buffer (P0013B, Beyotime, China), PBS (C0221A, Beyotime, China), Protease Inhibitor Cocktail (CW2200S, CWBIO, China), 5 × SDS loading buffer (CW0027S, CWBIO, China), TRIzon Reagent (CW0580, CWBIO, China), HiFiScript gDNA Removal RT MasterMix (CW2020M, CWBIO, China), protein A/G agarose (80104G, Invivogen, FR).Other reagents were the caspase inhibitor Z-VAD (HY-16658B, MedChemExpress, United States), Caspase-3 inhibitor Z-DEVD (HY-12466, MedChemExpress, United States), SYBR Green qPCR Mix (11201ES, YEASEN, China), Cell..

    Article Title: Infectious bursal disease virus affecting interferon regulatory factor 7 signaling through VP3 protein to facilitate viral replication
    Article Snippet: VP2 mouse monoclonal antibody (mAb) was prepared in our laboratory. .. Other antibodies used in our study were anti-IRF7 rabbit polyclonal antibody (pAb) (bs-2994R, BIOSS, China), anti-FLAG mouse mAb (CW0287, CWBIO, China), anti-β-actin mouse mAb (CW0096M, CWBIO, China), anti-His mouse mAb (M20001M, Abmart, China), AbBox Fluor 594-labeled goat anti-rabbit IgG (BD9279, Biodragon, China), FITC-labeled goat anti-mouse IgG (BF05001, Biodragon, China), goat anti-rabbit IgG H&L antibody (CW0103S, CWBIO, China), goat anti-mouse IgG H&L antibody (CW0102S, CWBIO, China). .. Other reagents were the proteasome inhibitor MG132 (A2585, APEXBIO, China), u

    Control:

    Article Title: Chicken caspase-3 promotes IBDV replication via the cleavage of IRF7
    Article Snippet: Anti-IBDV VP2 mouse monoclonal antibody (mAb) was prepared in our laboratory.Anti-IBDV VP2 mouse monoclonal antibody (mAb) was prepared in our laboratory.. Other antibodies used in our study were anti-IRF7 rabbit polyclonal antibody (pAb) (bs-2994R, BIOSS, China), anti-Caspase-3 (Pro and Active) mouse mAb (31A1067, Novus, United States), anti-β-actin mouse mAb (CW0096M, CWBIO, China), anti-His mouse mAb ( M20001 , Abmart, China), anti-HA mouse mAb ( M20003 , Abmart, China), goat anti-rabbit IgG H&L antibody (CW0103S, CWBIO, China), goat anti-mouse IgG H&L antibody (CW0102S, CWBIO, China), AbBox Fluor 594-labeled goat anti-rabbit IgG (BD9279, Biodragon, China), FITC-labeled goat anti-mouse IgG (BF05001, Biodragon, China).. Other reagents were the caspase inhibitor Z-VAD (HY-16658B, MedChemExpress, United States), Caspase-3 inhibitor Z-DEVD (HY-12466, MedChemExpress, United States), SYBR Green qPCR Mix (11201ES, YEASEN, China), Cell Counting Kit-8 (C6005, NCM Biotech, China), Annexin V-FITC Apoptosis Detection Kit (556547, BD Biosciences, United States), Lipo8000 transfection reagent (C0533, Beyotime, China), RIPA buffer (P0013B, Beyotime, China), PBS (C0221A, Beyotime, China), Protease Inhibitor Cocktail (CW2200S, CWBIO, China), 5 × SDS loading buffer (CW0027S, CWBIO, China), TRIzon Reagent (CW0580, CWBIO, China), HiFiScript gDNA Removal RT MasterMix (CW2020M, CWBIO, China), protein A/G agarose (80104G, Invivogen, FR).Other reagents were the caspase inhibitor Z-VAD (HY-16658B, MedChemExpress, United States), Caspase-3 inhibitor Z-DEVD (HY-12466, MedChemExpress, United States), SYBR Green qPCR Mix (11201ES, YEASEN, China), Cell..

    Article Title: Infectious bursal disease virus affecting interferon regulatory factor 7 signaling through VP3 protein to facilitate viral replication
    Article Snippet: VP2 mouse monoclonal antibody (mAb) was prepared in our laboratory. .. Other antibodies used in our study were anti-IRF7 rabbit polyclonal antibody (pAb) (bs-2994R, BIOSS, China), anti-FLAG mouse mAb (CW0287, CWBIO, China), anti-β-actin mouse mAb (CW0096M, CWBIO, China), anti-His mouse mAb (M20001M, Abmart, China), AbBox Fluor 594-labeled goat anti-rabbit IgG (BD9279, Biodragon, China), FITC-labeled goat anti-mouse IgG (BF05001, Biodragon, China), goat anti-rabbit IgG H&L antibody (CW0103S, CWBIO, China), goat anti-mouse IgG H&L antibody (CW0102S, CWBIO, China). .. Other reagents were the proteasome inhibitor MG132 (A2585, APEXBIO, China), u

    Immunoprecipitation:

    Article Title: Chicken caspase-3 promotes IBDV replication via the cleavage of IRF7
    Article Snippet: Anti-IBDV VP2 mouse monoclonal antibody (mAb) was prepared in our laboratory.Anti-IBDV VP2 mouse monoclonal antibody (mAb) was prepared in our laboratory.. Other antibodies used in our study were anti-IRF7 rabbit polyclonal antibody (pAb) (bs-2994R, BIOSS, China), anti-Caspase-3 (Pro and Active) mouse mAb (31A1067, Novus, United States), anti-β-actin mouse mAb (CW0096M, CWBIO, China), anti-His mouse mAb ( M20001 , Abmart, China), anti-HA mouse mAb ( M20003 , Abmart, China), goat anti-rabbit IgG H&L antibody (CW0103S, CWBIO, China), goat anti-mouse IgG H&L antibody (CW0102S, CWBIO, China), AbBox Fluor 594-labeled goat anti-rabbit IgG (BD9279, Biodragon, China), FITC-labeled goat anti-mouse IgG (BF05001, Biodragon, China).. Other reagents were the caspase inhibitor Z-VAD (HY-16658B, MedChemExpress, United States), Caspase-3 inhibitor Z-DEVD (HY-12466, MedChemExpress, United States), SYBR Green qPCR Mix (11201ES, YEASEN, China), Cell Counting Kit-8 (C6005, NCM Biotech, China), Annexin V-FITC Apoptosis Detection Kit (556547, BD Biosciences, United States), Lipo8000 transfection reagent (C0533, Beyotime, China), RIPA buffer (P0013B, Beyotime, China), PBS (C0221A, Beyotime, China), Protease Inhibitor Cocktail (CW2200S, CWBIO, China), 5 × SDS loading buffer (CW0027S, CWBIO, China), TRIzon Reagent (CW0580, CWBIO, China), HiFiScript gDNA Removal RT MasterMix (CW2020M, CWBIO, China), protein A/G agarose (80104G, Invivogen, FR).Other reagents were the caspase inhibitor Z-VAD (HY-16658B, MedChemExpress, United States), Caspase-3 inhibitor Z-DEVD (HY-12466, MedChemExpress, United States), SYBR Green qPCR Mix (11201ES, YEASEN, China), Cell..

    Article Title: Infectious bursal disease virus affecting interferon regulatory factor 7 signaling through VP3 protein to facilitate viral replication
    Article Snippet: VP2 mouse monoclonal antibody (mAb) was prepared in our laboratory. .. Other antibodies used in our study were anti-IRF7 rabbit polyclonal antibody (pAb) (bs-2994R, BIOSS, China), anti-FLAG mouse mAb (CW0287, CWBIO, China), anti-β-actin mouse mAb (CW0096M, CWBIO, China), anti-His mouse mAb (M20001M, Abmart, China), AbBox Fluor 594-labeled goat anti-rabbit IgG (BD9279, Biodragon, China), FITC-labeled goat anti-mouse IgG (BF05001, Biodragon, China), goat anti-rabbit IgG H&L antibody (CW0103S, CWBIO, China), goat anti-mouse IgG H&L antibody (CW0102S, CWBIO, China). .. Other reagents were the proteasome inhibitor MG132 (A2585, APEXBIO, China), u

    FLAG-tag:

    Article Title: Chicken caspase-3 promotes IBDV replication via the cleavage of IRF7
    Article Snippet: Anti-IBDV VP2 mouse monoclonal antibody (mAb) was prepared in our laboratory.Anti-IBDV VP2 mouse monoclonal antibody (mAb) was prepared in our laboratory.. Other antibodies used in our study were anti-IRF7 rabbit polyclonal antibody (pAb) (bs-2994R, BIOSS, China), anti-Caspase-3 (Pro and Active) mouse mAb (31A1067, Novus, United States), anti-β-actin mouse mAb (CW0096M, CWBIO, China), anti-His mouse mAb ( M20001 , Abmart, China), anti-HA mouse mAb ( M20003 , Abmart, China), goat anti-rabbit IgG H&L antibody (CW0103S, CWBIO, China), goat anti-mouse IgG H&L antibody (CW0102S, CWBIO, China), AbBox Fluor 594-labeled goat anti-rabbit IgG (BD9279, Biodragon, China), FITC-labeled goat anti-mouse IgG (BF05001, Biodragon, China).. Other reagents were the caspase inhibitor Z-VAD (HY-16658B, MedChemExpress, United States), Caspase-3 inhibitor Z-DEVD (HY-12466, MedChemExpress, United States), SYBR Green qPCR Mix (11201ES, YEASEN, China), Cell Counting Kit-8 (C6005, NCM Biotech, China), Annexin V-FITC Apoptosis Detection Kit (556547, BD Biosciences, United States), Lipo8000 transfection reagent (C0533, Beyotime, China), RIPA buffer (P0013B, Beyotime, China), PBS (C0221A, Beyotime, China), Protease Inhibitor Cocktail (CW2200S, CWBIO, China), 5 × SDS loading buffer (CW0027S, CWBIO, China), TRIzon Reagent (CW0580, CWBIO, China), HiFiScript gDNA Removal RT MasterMix (CW2020M, CWBIO, China), protein A/G agarose (80104G, Invivogen, FR).Other reagents were the caspase inhibitor Z-VAD (HY-16658B, MedChemExpress, United States), Caspase-3 inhibitor Z-DEVD (HY-12466, MedChemExpress, United States), SYBR Green qPCR Mix (11201ES, YEASEN, China), Cell..

    Article Title: Infectious bursal disease virus affecting interferon regulatory factor 7 signaling through VP3 protein to facilitate viral replication
    Article Snippet: VP2 mouse monoclonal antibody (mAb) was prepared in our laboratory. .. Other antibodies used in our study were anti-IRF7 rabbit polyclonal antibody (pAb) (bs-2994R, BIOSS, China), anti-FLAG mouse mAb (CW0287, CWBIO, China), anti-β-actin mouse mAb (CW0096M, CWBIO, China), anti-His mouse mAb (M20001M, Abmart, China), AbBox Fluor 594-labeled goat anti-rabbit IgG (BD9279, Biodragon, China), FITC-labeled goat anti-mouse IgG (BF05001, Biodragon, China), goat anti-rabbit IgG H&L antibody (CW0103S, CWBIO, China), goat anti-mouse IgG H&L antibody (CW0102S, CWBIO, China). .. Other reagents were the proteasome inhibitor MG132 (A2585, APEXBIO, China), u

    Immunofluorescence:

    Article Title: Chicken caspase-3 promotes IBDV replication via the cleavage of IRF7
    Article Snippet: Anti-IBDV VP2 mouse monoclonal antibody (mAb) was prepared in our laboratory.Anti-IBDV VP2 mouse monoclonal antibody (mAb) was prepared in our laboratory.. Other antibodies used in our study were anti-IRF7 rabbit polyclonal antibody (pAb) (bs-2994R, BIOSS, China), anti-Caspase-3 (Pro and Active) mouse mAb (31A1067, Novus, United States), anti-β-actin mouse mAb (CW0096M, CWBIO, China), anti-His mouse mAb ( M20001 , Abmart, China), anti-HA mouse mAb ( M20003 , Abmart, China), goat anti-rabbit IgG H&L antibody (CW0103S, CWBIO, China), goat anti-mouse IgG H&L antibody (CW0102S, CWBIO, China), AbBox Fluor 594-labeled goat anti-rabbit IgG (BD9279, Biodragon, China), FITC-labeled goat anti-mouse IgG (BF05001, Biodragon, China).. Other reagents were the caspase inhibitor Z-VAD (HY-16658B, MedChemExpress, United States), Caspase-3 inhibitor Z-DEVD (HY-12466, MedChemExpress, United States), SYBR Green qPCR Mix (11201ES, YEASEN, China), Cell Counting Kit-8 (C6005, NCM Biotech, China), Annexin V-FITC Apoptosis Detection Kit (556547, BD Biosciences, United States), Lipo8000 transfection reagent (C0533, Beyotime, China), RIPA buffer (P0013B, Beyotime, China), PBS (C0221A, Beyotime, China), Protease Inhibitor Cocktail (CW2200S, CWBIO, China), 5 × SDS loading buffer (CW0027S, CWBIO, China), TRIzon Reagent (CW0580, CWBIO, China), HiFiScript gDNA Removal RT MasterMix (CW2020M, CWBIO, China), protein A/G agarose (80104G, Invivogen, FR).Other reagents were the caspase inhibitor Z-VAD (HY-16658B, MedChemExpress, United States), Caspase-3 inhibitor Z-DEVD (HY-12466, MedChemExpress, United States), SYBR Green qPCR Mix (11201ES, YEASEN, China), Cell..

    Article Title: Infectious bursal disease virus affecting interferon regulatory factor 7 signaling through VP3 protein to facilitate viral replication
    Article Snippet: VP2 mouse monoclonal antibody (mAb) was prepared in our laboratory. .. Other antibodies used in our study were anti-IRF7 rabbit polyclonal antibody (pAb) (bs-2994R, BIOSS, China), anti-FLAG mouse mAb (CW0287, CWBIO, China), anti-β-actin mouse mAb (CW0096M, CWBIO, China), anti-His mouse mAb (M20001M, Abmart, China), AbBox Fluor 594-labeled goat anti-rabbit IgG (BD9279, Biodragon, China), FITC-labeled goat anti-mouse IgG (BF05001, Biodragon, China), goat anti-rabbit IgG H&L antibody (CW0103S, CWBIO, China), goat anti-mouse IgG H&L antibody (CW0102S, CWBIO, China). .. Other reagents were the proteasome inhibitor MG132 (A2585, APEXBIO, China), u

    Staining:

    Article Title: Chicken caspase-3 promotes IBDV replication via the cleavage of IRF7
    Article Snippet: Anti-IBDV VP2 mouse monoclonal antibody (mAb) was prepared in our laboratory.Anti-IBDV VP2 mouse monoclonal antibody (mAb) was prepared in our laboratory.. Other antibodies used in our study were anti-IRF7 rabbit polyclonal antibody (pAb) (bs-2994R, BIOSS, China), anti-Caspase-3 (Pro and Active) mouse mAb (31A1067, Novus, United States), anti-β-actin mouse mAb (CW0096M, CWBIO, China), anti-His mouse mAb ( M20001 , Abmart, China), anti-HA mouse mAb ( M20003 , Abmart, China), goat anti-rabbit IgG H&L antibody (CW0103S, CWBIO, China), goat anti-mouse IgG H&L antibody (CW0102S, CWBIO, China), AbBox Fluor 594-labeled goat anti-rabbit IgG (BD9279, Biodragon, China), FITC-labeled goat anti-mouse IgG (BF05001, Biodragon, China).. Other reagents were the caspase inhibitor Z-VAD (HY-16658B, MedChemExpress, United States), Caspase-3 inhibitor Z-DEVD (HY-12466, MedChemExpress, United States), SYBR Green qPCR Mix (11201ES, YEASEN, China), Cell Counting Kit-8 (C6005, NCM Biotech, China), Annexin V-FITC Apoptosis Detection Kit (556547, BD Biosciences, United States), Lipo8000 transfection reagent (C0533, Beyotime, China), RIPA buffer (P0013B, Beyotime, China), PBS (C0221A, Beyotime, China), Protease Inhibitor Cocktail (CW2200S, CWBIO, China), 5 × SDS loading buffer (CW0027S, CWBIO, China), TRIzon Reagent (CW0580, CWBIO, China), HiFiScript gDNA Removal RT MasterMix (CW2020M, CWBIO, China), protein A/G agarose (80104G, Invivogen, FR).Other reagents were the caspase inhibitor Z-VAD (HY-16658B, MedChemExpress, United States), Caspase-3 inhibitor Z-DEVD (HY-12466, MedChemExpress, United States), SYBR Green qPCR Mix (11201ES, YEASEN, China), Cell..

    Article Title: Infectious bursal disease virus affecting interferon regulatory factor 7 signaling through VP3 protein to facilitate viral replication
    Article Snippet: VP2 mouse monoclonal antibody (mAb) was prepared in our laboratory. .. Other antibodies used in our study were anti-IRF7 rabbit polyclonal antibody (pAb) (bs-2994R, BIOSS, China), anti-FLAG mouse mAb (CW0287, CWBIO, China), anti-β-actin mouse mAb (CW0096M, CWBIO, China), anti-His mouse mAb (M20001M, Abmart, China), AbBox Fluor 594-labeled goat anti-rabbit IgG (BD9279, Biodragon, China), FITC-labeled goat anti-mouse IgG (BF05001, Biodragon, China), goat anti-rabbit IgG H&L antibody (CW0103S, CWBIO, China), goat anti-mouse IgG H&L antibody (CW0102S, CWBIO, China). .. Other reagents were the proteasome inhibitor MG132 (A2585, APEXBIO, China), u



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    Figure 2 The 3CD protein interacts with IRF7 protein and inhibits the expression of IRF7 protein.A pCAGGS-3CD-HA and pCAGGS-IRF7-Flag were co-transfected or both were separately co-transfected with pCAGGS into 24-well plates (transfection ratio of 1:1), and cell samples were collected 36 h after transfection for indirect immunofluorescence experiments to observe the intracellular localization of the 3CD protein and IRF7 protein. B Cells were grown in 6-well plates and each well was transfected with a total of 1 µg pCAGGS-3CD-HA and 1 µg pCAGGS-IRF7-Flag. Cells were lysed 36 h after transfection, and lysates were immunoprecipitated with mouse anti-Flag or mouse IgG antibodies and subjected to Western blotting. C 0.4 µg pCAGGS-IRF7-Flag was co-transfected into 12-well plates with 0.6 µg pCAGGS-3CD-HA, pCAGGS-3D-HA, pCMV-3 C-HA or empty, respectively, and cell samples were collected 36 h after transfection to detect the expression of IRF7 protein. D Similar transfection and quantitative PCR experiments were performed to analyze the effect of 3CD protein on Poly(I:C)-induced IRF7 mRNA levels as described in Figure 1A. * P < 0.05, ** P < 0.01, *** P < 0.001, compared with the control group. E DEFs were grown in 12-well plates, and different doses of 3CD-HA expression plasmids were transferred into the cells (the amount transfected into each group was 1 µg, and pCAGGS was used to supplement the insufficient group). The expression of 3CD protein and endogenous IRF7 protein was detected by Western blotting at 36 h. F DEFs were grown in 12-well plates, and 0.4 µg of pCAGGS-IRF7-Flag was co-transfected with different doses of pCAGGS-3CD-HA (the amount transfected into each group was 1 µg, and pCAGGS was used to supplement the insufficient group). 36 h after transfection, cell samples were collected, and the protein expressions of 3CD and IRF7 were detected.

    Journal: Veterinary research

    Article Title: DHAV 3CD targets IRF7 and RIG-I proteins to block the type I interferon upstream signaling pathway.

    doi: 10.1186/s13567-023-01134-4

    Figure Lengend Snippet: Figure 2 The 3CD protein interacts with IRF7 protein and inhibits the expression of IRF7 protein.A pCAGGS-3CD-HA and pCAGGS-IRF7-Flag were co-transfected or both were separately co-transfected with pCAGGS into 24-well plates (transfection ratio of 1:1), and cell samples were collected 36 h after transfection for indirect immunofluorescence experiments to observe the intracellular localization of the 3CD protein and IRF7 protein. B Cells were grown in 6-well plates and each well was transfected with a total of 1 µg pCAGGS-3CD-HA and 1 µg pCAGGS-IRF7-Flag. Cells were lysed 36 h after transfection, and lysates were immunoprecipitated with mouse anti-Flag or mouse IgG antibodies and subjected to Western blotting. C 0.4 µg pCAGGS-IRF7-Flag was co-transfected into 12-well plates with 0.6 µg pCAGGS-3CD-HA, pCAGGS-3D-HA, pCMV-3 C-HA or empty, respectively, and cell samples were collected 36 h after transfection to detect the expression of IRF7 protein. D Similar transfection and quantitative PCR experiments were performed to analyze the effect of 3CD protein on Poly(I:C)-induced IRF7 mRNA levels as described in Figure 1A. * P < 0.05, ** P < 0.01, *** P < 0.001, compared with the control group. E DEFs were grown in 12-well plates, and different doses of 3CD-HA expression plasmids were transferred into the cells (the amount transfected into each group was 1 µg, and pCAGGS was used to supplement the insufficient group). The expression of 3CD protein and endogenous IRF7 protein was detected by Western blotting at 36 h. F DEFs were grown in 12-well plates, and 0.4 µg of pCAGGS-IRF7-Flag was co-transfected with different doses of pCAGGS-3CD-HA (the amount transfected into each group was 1 µg, and pCAGGS was used to supplement the insufficient group). 36 h after transfection, cell samples were collected, and the protein expressions of 3CD and IRF7 were detected.

    Article Snippet: A mouse anti-Flag monoclonal antibody (Cat: M185-3 S) and a mouse antiHA monoclonal antibody (Cat: M132-3) were purchased from Medical & Biological Laboratories Co., Ltd. A rabbit anti-duck IRF7 polyclonal antibody, a HRP-conjugated goat anti-mouse IgG heavy chain antibody (Cat: AS064), and a HRP-conjugated goat anti-mouse IgG light chain antibody (Cat: AS062) were prepared by ABclonal Technology Co., Ltd. A rabbit anti-beta (β)-actin antibody (Cat: 20536-1-AP) was obtained from Proteintech Co., Ltd. A rabbit anti-HA monoclonal antibody (Cat: AF2305), a mouse IgG antibody (Cat: A7028), and a HRP-conjugated goat anti-mouse IgG (Cat: A0216) were purchased from Beyotime Co., Ltd. A rabbit anti-Histone H3 (Cat: TA6358), a mouse anti-Myc (Cat: T62076M), and a mouse anti-beta (β)-tubulin monoclonal antibody (Cat: T63017-2) were purchased from Abmart Co., Ltd. A rabbit anti-VP3 antibody was prepared in our laboratory [19].

    Techniques: Expressing, Transfection, Immunofluorescence, Immunoprecipitation, Western Blot, Real-time Polymerase Chain Reaction, Control

    Figure 3 Effect of the 3CD protein on the nuclear translocation of the IRF7 protein.A Three experimental groups were set up, both groups I and II were co-transfected with 0.3 µg pCAGGS-IRF7-Flag and 0.5 µg pCAGGS; the third group was transfected with 0.3 µg pCAGGS-IRF7-Flag and 0.5 µg pCAGGS-3CD-HA, and transfect the plasmids into cells in a 24-well plate. 24 h after transfection groups II and III were stimulated with 5 µg/µL Poly (I:C) for 12 h, and cell samples were collected for indirect immunofluorescence experiments. B Images such as those in panel A were subjected to image analysis (see Materials and methods), where > 30 cells for each sample were analyzed to determine Fn/c, the fluorescence in the nucleus relative to that in the cytoplasm. Results are means ± standard errors of the mean (n ≥ 30). C DEFs were cultured in 6-well plates, grouped and transfected as described above for group A (transfection ratio of 3:2), 24 h after transfection groups 2 and 3 were stimulated with 20 µg/µL Poly(I:C) for 12 h, and cell samples were collected for Western blot analysis. D Gray value analysis was performed on the bands in C with ImageJ software to determine the ratio of Pn/c: Pn/c = Pn/Pc, Pn (relative protein level of IRF7 in the nucleus) = gray value of nuclear IRF7 protein/ Histone H3.1, Pc (relative protein level of IRF7 in the cytoplasm) = gray value of cytoplasmic IRF7 protein/β-tubulin. Where the data in each group were normalized relative to the control group. Values represent the mean (± SD) from three independent experiments. NS: not significant, * P < 0.05, ** P < 0.01, *** P < 0.001, compared with the control group.

    Journal: Veterinary research

    Article Title: DHAV 3CD targets IRF7 and RIG-I proteins to block the type I interferon upstream signaling pathway.

    doi: 10.1186/s13567-023-01134-4

    Figure Lengend Snippet: Figure 3 Effect of the 3CD protein on the nuclear translocation of the IRF7 protein.A Three experimental groups were set up, both groups I and II were co-transfected with 0.3 µg pCAGGS-IRF7-Flag and 0.5 µg pCAGGS; the third group was transfected with 0.3 µg pCAGGS-IRF7-Flag and 0.5 µg pCAGGS-3CD-HA, and transfect the plasmids into cells in a 24-well plate. 24 h after transfection groups II and III were stimulated with 5 µg/µL Poly (I:C) for 12 h, and cell samples were collected for indirect immunofluorescence experiments. B Images such as those in panel A were subjected to image analysis (see Materials and methods), where > 30 cells for each sample were analyzed to determine Fn/c, the fluorescence in the nucleus relative to that in the cytoplasm. Results are means ± standard errors of the mean (n ≥ 30). C DEFs were cultured in 6-well plates, grouped and transfected as described above for group A (transfection ratio of 3:2), 24 h after transfection groups 2 and 3 were stimulated with 20 µg/µL Poly(I:C) for 12 h, and cell samples were collected for Western blot analysis. D Gray value analysis was performed on the bands in C with ImageJ software to determine the ratio of Pn/c: Pn/c = Pn/Pc, Pn (relative protein level of IRF7 in the nucleus) = gray value of nuclear IRF7 protein/ Histone H3.1, Pc (relative protein level of IRF7 in the cytoplasm) = gray value of cytoplasmic IRF7 protein/β-tubulin. Where the data in each group were normalized relative to the control group. Values represent the mean (± SD) from three independent experiments. NS: not significant, * P < 0.05, ** P < 0.01, *** P < 0.001, compared with the control group.

    Article Snippet: A mouse anti-Flag monoclonal antibody (Cat: M185-3 S) and a mouse antiHA monoclonal antibody (Cat: M132-3) were purchased from Medical & Biological Laboratories Co., Ltd. A rabbit anti-duck IRF7 polyclonal antibody, a HRP-conjugated goat anti-mouse IgG heavy chain antibody (Cat: AS064), and a HRP-conjugated goat anti-mouse IgG light chain antibody (Cat: AS062) were prepared by ABclonal Technology Co., Ltd. A rabbit anti-beta (β)-actin antibody (Cat: 20536-1-AP) was obtained from Proteintech Co., Ltd. A rabbit anti-HA monoclonal antibody (Cat: AF2305), a mouse IgG antibody (Cat: A7028), and a HRP-conjugated goat anti-mouse IgG (Cat: A0216) were purchased from Beyotime Co., Ltd. A rabbit anti-Histone H3 (Cat: TA6358), a mouse anti-Myc (Cat: T62076M), and a mouse anti-beta (β)-tubulin monoclonal antibody (Cat: T63017-2) were purchased from Abmart Co., Ltd. A rabbit anti-VP3 antibody was prepared in our laboratory [19].

    Techniques: Translocation Assay, Transfection, Immunofluorescence, Fluorescence, Cell Culture, Western Blot, Software, Control

    Figure 9 A model of DHAV-1-mediated inhibition of IFNβ signaling pathway. After DHAV-1 infection, both viral proteins 3 C and 3CD can inhibit the production of IFNβ by interacting with IRF7 protein and inhibiting the expression of IRF7 protein, and the 3 C protein also inhibits the nuclear translocation of the IRF7 protein, blocking interferon signaling. In addition, the 3CD protein can interact with RIG-I to inhibit its expression, and can also interfere with the formation of a complex between RIG-I and MAVS to ultimately inhibit the RIG-I-mediated signaling pathway.

    Journal: Veterinary research

    Article Title: DHAV 3CD targets IRF7 and RIG-I proteins to block the type I interferon upstream signaling pathway.

    doi: 10.1186/s13567-023-01134-4

    Figure Lengend Snippet: Figure 9 A model of DHAV-1-mediated inhibition of IFNβ signaling pathway. After DHAV-1 infection, both viral proteins 3 C and 3CD can inhibit the production of IFNβ by interacting with IRF7 protein and inhibiting the expression of IRF7 protein, and the 3 C protein also inhibits the nuclear translocation of the IRF7 protein, blocking interferon signaling. In addition, the 3CD protein can interact with RIG-I to inhibit its expression, and can also interfere with the formation of a complex between RIG-I and MAVS to ultimately inhibit the RIG-I-mediated signaling pathway.

    Article Snippet: A mouse anti-Flag monoclonal antibody (Cat: M185-3 S) and a mouse antiHA monoclonal antibody (Cat: M132-3) were purchased from Medical & Biological Laboratories Co., Ltd. A rabbit anti-duck IRF7 polyclonal antibody, a HRP-conjugated goat anti-mouse IgG heavy chain antibody (Cat: AS064), and a HRP-conjugated goat anti-mouse IgG light chain antibody (Cat: AS062) were prepared by ABclonal Technology Co., Ltd. A rabbit anti-beta (β)-actin antibody (Cat: 20536-1-AP) was obtained from Proteintech Co., Ltd. A rabbit anti-HA monoclonal antibody (Cat: AF2305), a mouse IgG antibody (Cat: A7028), and a HRP-conjugated goat anti-mouse IgG (Cat: A0216) were purchased from Beyotime Co., Ltd. A rabbit anti-Histone H3 (Cat: TA6358), a mouse anti-Myc (Cat: T62076M), and a mouse anti-beta (β)-tubulin monoclonal antibody (Cat: T63017-2) were purchased from Abmart Co., Ltd. A rabbit anti-VP3 antibody was prepared in our laboratory [19].

    Techniques: Inhibition, Infection, Expressing, Translocation Assay, Blocking Assay